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gltC [2017-03-18 10:04:45]
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gltC [2017-03-18 10:04:45]

Transcriptional activator of the gltA-gltB operon. Activates expression of the operon in the absence of arginine.
locus
BSU18460
pI
5.62
mw
33.90 kDa
protein length
300 aa Sequence Blast
gene length
900 bp Sequence Blast
function
positive regulation
of the glutamate synthase operon (gltAB)
product
transcriptional regulator (LysR family)
essential
No
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.1|Biosynthesis/ acquisition of glutamate/ glutamine/ ammonium assimilation]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]

    • Gene

    Coordinates on the chromosome (coding sequence)
    2,014,779 -> 2,015,681

    Phenotypes of a mutant

  • ''gltC'' mutants are auxotrophic for glutamate.

    • The protein

    Catalyzed reaction/ biological activity

  • transcription activation of the ''[gene|44DF1B7E476AD58B3CCC39300FFE0132D0D32AD0|gltA]-[gene|AA07CC52B2DD48ACC9D3375E9D531CB1ACBE485A|gltB]'' operon [Pubmed|2548995]

    • Protein family

  • [SW|LysR family] [Pubmed|2548995]

    • Paralogous protein(s)

  • none, but there are 19 members of the LysR family in ''B. subtilis

    • [SW|Domains]

  • DNA-binding helix-turn-helix motif: AA 18 ... 37

    • Effectors of protein activity

  • 2-oxoglutarate stimulates transcription activation, glutamate inhibits transcription activation [Pubmed|17134717]
    • Expression and Regulation
    [[this]]

    Biological materials

    Mutant

  • GP344 (erm), (available in [SW|Jörg Stülke]'s lab)
  • GP738 (spc), (available in [SW|Jörg Stülke]'s lab)
  • GP1904 (''gltC''::''aphA3''), (available in [SW|Jörg Stülke]'s lab) [pubmed|28294562]

    • Expression vector

  • for expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP903, available in [SW|Jörg Stülke]'s lab
  • for expression, purification in ''E. coli'' with C-terminal Strep-tag, in pET3C: pGP951, available in [SW|Jörg Stülke]'s lab

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • Antibody

  • available in [SW|Jörg Stülke]'s lab

    • Labs working on this gene/protein

  • [SW|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]
  • [SW|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
  • [SW|Fabian Commichau] University of Göttingen, Germany [http://genmibio.uni-goettingen.de/index.php?id=130 Homepage]

    • References

    Reviews

  • 20408793,22625175

    • Original Publications

  • 7559360,15150225,2548995,17183217,17608797,17134717,14523131,20630473,25711804,28294562